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Evaluation of LIPT1 expression levels in chRCC. (A) Analysis of LIPT1 expression in chRCC in TCGA database. (B) <t>qPCR</t> detection of LIPT1 mRNA expression level in human renal epithelial cells (HKb20) and human chRCC cells (RCC98) ( n = 3 per group). (C) WB experiments were conducted to examine the expression level of LIPT1 protein in human renal epithelial cells (HKb20) and human chRCC cells (RCC98) ( n = 3 per group). * P -values <0.05: statistically significant.
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Evaluation of LIPT1 expression levels in chRCC. (A) Analysis of LIPT1 expression in chRCC in TCGA database. (B) <t>qPCR</t> detection of LIPT1 mRNA expression level in human renal epithelial cells (HKb20) and human chRCC cells (RCC98) ( n = 3 per group). (C) WB experiments were conducted to examine the expression level of LIPT1 protein in human renal epithelial cells (HKb20) and human chRCC cells (RCC98) ( n = 3 per group). * P -values <0.05: statistically significant.
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Evaluation of LIPT1 expression levels in chRCC. (A) Analysis of LIPT1 expression in chRCC in TCGA database. (B) <t>qPCR</t> detection of LIPT1 mRNA expression level in human renal epithelial cells (HKb20) and human chRCC cells (RCC98) ( n = 3 per group). (C) WB experiments were conducted to examine the expression level of LIPT1 protein in human renal epithelial cells (HKb20) and human chRCC cells (RCC98) ( n = 3 per group). * P -values <0.05: statistically significant.
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Evaluation of LIPT1 expression levels in chRCC. (A) Analysis of LIPT1 expression in chRCC in TCGA database. (B) qPCR detection of LIPT1 mRNA expression level in human renal epithelial cells (HKb20) and human chRCC cells (RCC98) ( n = 3 per group). (C) WB experiments were conducted to examine the expression level of LIPT1 protein in human renal epithelial cells (HKb20) and human chRCC cells (RCC98) ( n = 3 per group). * P -values <0.05: statistically significant.

Journal: Briefings in Functional Genomics

Article Title: Effect of EGR1/LIPT1 regulatory axis on cuproptosis in chromophobe renal cell carcinoma

doi: 10.1093/bfgp/elaf023

Figure Lengend Snippet: Evaluation of LIPT1 expression levels in chRCC. (A) Analysis of LIPT1 expression in chRCC in TCGA database. (B) qPCR detection of LIPT1 mRNA expression level in human renal epithelial cells (HKb20) and human chRCC cells (RCC98) ( n = 3 per group). (C) WB experiments were conducted to examine the expression level of LIPT1 protein in human renal epithelial cells (HKb20) and human chRCC cells (RCC98) ( n = 3 per group). * P -values <0.05: statistically significant.

Article Snippet: Quantitative polymerase chain reaction (qPCR) was conducted on cDNA, with SYBR Green qPCR detection kit (Toyobo, Japan) used and β-actin as the reference gene.

Techniques: Expressing

Verification of EGR1’s ability to transcriptionally activate LIPT1. (A) hTFtarget predicted potential upstream TFs of LIPT1. (B) Bioinformatics analysis analyzed Pearson correlation between EGR1 and LIPT1. (C) EGR1 expression levels in chRCC tissues in TCGA database. (D) TASPAR predicted binding sites for EGR1 and LIPT1. (E) qPCR verified transfection efficiency ( n = 3 per group). (F) Dual-luciferase assay verified the binding relationship between EGR1 and LIPT1 ( n = 3 per group). (G) The CHIP experiment verified the target binding relationship between EGR1 and LIPT1 ( n = 3 per group). (H) qPCR was used to detect the content of LIPT1 mRNA in both groups ( n = 3 per group). (I) WB test was used to detect the expression of LIPT1 protein in the two groups ( n = 3 per group). ns indicates no significant difference; * indicates P < 0.05.

Journal: Briefings in Functional Genomics

Article Title: Effect of EGR1/LIPT1 regulatory axis on cuproptosis in chromophobe renal cell carcinoma

doi: 10.1093/bfgp/elaf023

Figure Lengend Snippet: Verification of EGR1’s ability to transcriptionally activate LIPT1. (A) hTFtarget predicted potential upstream TFs of LIPT1. (B) Bioinformatics analysis analyzed Pearson correlation between EGR1 and LIPT1. (C) EGR1 expression levels in chRCC tissues in TCGA database. (D) TASPAR predicted binding sites for EGR1 and LIPT1. (E) qPCR verified transfection efficiency ( n = 3 per group). (F) Dual-luciferase assay verified the binding relationship between EGR1 and LIPT1 ( n = 3 per group). (G) The CHIP experiment verified the target binding relationship between EGR1 and LIPT1 ( n = 3 per group). (H) qPCR was used to detect the content of LIPT1 mRNA in both groups ( n = 3 per group). (I) WB test was used to detect the expression of LIPT1 protein in the two groups ( n = 3 per group). ns indicates no significant difference; * indicates P < 0.05.

Article Snippet: Quantitative polymerase chain reaction (qPCR) was conducted on cDNA, with SYBR Green qPCR detection kit (Toyobo, Japan) used and β-actin as the reference gene.

Techniques: Expressing, Binding Assay, Transfection, Luciferase